Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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R&D Systems™ Human TNF-alpha Quantikine ELISA Kit
Human TNF-alpha Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
R&D Systems™ Human CTGF/CCN2 DuoSet ELISA
Human CTGF/CCN2 DuoSet ELISA from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
R&D Systems™ Endothelin-1 Quantikine ELISA Kit
Endothelin-1 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
| Specificity | Synthetic and Natural Endothelin-1 |
|---|---|
| Target Species | Multi-species |
| Conjugate | HRP |
| Product Type | ELISA Kit |
| Sample Type | Cell Culture Supernatant,Plasma (EDTA, Heparin),Serum,Urine |
| Sample Volume | Cell Culture Supernates (75uL),Serum (75uL),EDTA Plasma (75uL),Heparin Plasma (75uL),Urine (75uL) |
| Synonym | ARCND3, EDN1, endothelin 1, endothelin-1, ET1, HDLCQ7, PPET1, Preproendothelin-1, QME |
| Assay Sensitivity | 0.207pg/mL |
| Storage Requirements | Store the unopened product at 2° to 8°C. Do not use past expiration date. |
| Assay Range | 0.39 - 25pg/mL |
| For Use With (Application) | ELISA |
| Cross Reactivity | <50% cross-species reactivity observed with species tested. Cross-reactivity observed with 1 or more available related molecules. |
R&D Systems™ Human IL-12 p70 Quantikine ELISA Kit
Human IL-12 p70 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
| Specificity | Natural and Recombinant Human IL-12p70 |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Product Type | Solid Phase Sandwich ELISA |
| Gene ID (Entrez) | 3592 |
| Sample Type | Cell Culture Supernatant,Plasma (EDTA, Citrate, Heparin),Serum,Urine |
| Sample Volume | Cell Culture Supernates (200uL),Serum (200uL),EDTA Plasma (200uL),Heparin Plasma (200uL),Citrate Plasma (200uL),Urine (200uL) |
| Synonym | CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12 A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35 |
| Assay Sensitivity | 5pg/mL |
| Storage Requirements | Store the unopened product at 2° to 8°C. Do not use past expiration date. |
| Assay Range | 7.80 - 500pg/mL |
| For Use With (Application) | ELISA |
| Cross Reactivity | <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules |
Invitrogen™ Human TNF alpha (Total) ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 8.1% |
| Intraassay CV | 7.7 |
| Gene Symbol | DIF-alpha,TNFA,TNFSF2,TNLG1F,TNF |
| Total Assay Time | 3 hrs 10 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 5.0 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 23 to 1,500 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 20 mins |
| Accession Number | P01375 |
| Regulatory Status | RUO |
| Target | TNF alpha |
| Kit Contents | Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 7124 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | Cachectin, Tumor necrosis factor alpha, DIF |
R&D Systems™ Mouse S100A8/S100A9 Heterodimer DuoSet ELISA
Mouse S100A8/S100A9 Heterodimer DuoSet ELISA from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
Invitrogen™ Human IL-10 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Measurement | Absorbance at 450 nm minus absorbance (at 550 nm) |
|---|---|
| Immunoassay Kit Format | Colorimetric ELISA (TMB substrate) with Plates |
| Target Species | Human |
| Conjugate | HRP |
| Description | Human IL-10 ELISA Kit |
| Interassay CV | <10% |
| Intraassay CV | <10 |
| Gene Symbol | CSIF,GVHDS,IL-10 A,TGIF,IL10 |
| Total Assay Time | 3 hrs |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 50 μL |
| Assay Sensitivity | <3 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 15.36 to 600 pg/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | P22301 |
| Regulatory Status | RUO |
| Target | IL-10 |
| Specificity | No significant cross-reaction with human IL-1 A, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, TNFß or IFNg. Approx. 2% cross-reactivity with recombinant mouse IL-10 |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| Precision | Intra-assay CV <10%; inter-assay CV <10% |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3586 |
| Sample Type | Plasma,Serum,Supernatant,Urine |
| Label or Dye | HRP |
Invitrogen™ Mouse S100A8/S100A9 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | S100A8, S100A9 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.65 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.65 to 150 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | 60B8Ag; AI323541; AW546964; B8Ag; BEE11; BEE22; Caga; Cagb; calgranulin A; calgranulin B; calgranulin-A; Calgranulin-B; calprotectin; calprotectin L1H subunit; Calprotectin L1L subunit; CFAG; CGLA; CGLB; Chemotactic cytokine CP-10; chemotactic S100 protein; CP-10; Cystic fibrosis antigen; EGK |
| Regulatory Status | RUO |
| Target | Calprotectin |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 20201, 20202 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
R&D Systems™ Mouse/Rat Cystatin C Quantikine ELISA Kit
Mouse/Rat Cystatin C Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
Invitrogen™ Human SARS-CoV-2 Spike (Trimer) IgG ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <10% |
| Intraassay CV | <10 |
| Total Assay Time | 1 hrs 15 mins |
| Sample Volume | Plasma 5 μL, Serum 5 μL |
| Assay Sensitivity | <39 Units/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 234 to 15,000 Units/mL |
| Test Time | 30 mins |
| Regulatory Status | RUO |
| Target | SARS-CoV-2 Spike (trimer) Protein |
| Kit Contents | Pre-coated 96 well plate,High Control (standard),Calibrator (medium control),Low Control,IgG - HRP conjugate,Assay Buffer concentrate,Wash Buffer concentrate,Substrate solution,Stop Solution,Adhesive Plate Covers |
| Gene Alias | Coronavirus; COVID; Covid 19; COVID19; COVID-19; SARS; SARS Coronavirus; SARS CoV; SARS CoV2; SARS CoV-2; SARS Spike Protein; SARS-CoV-2 |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Sample Type | Plasma,Serum |
| Label or Dye | HRP |
Invitrogen™ Mouse Amyloid beta 40 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Superoxide Dismutase (SOD) Colorimetric Activity Kit
Assay Superoxide Dismutase (SOD) Activity in Serum, Plasma, Cells, Tissues and Erythrocyte Lysates.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Approved for shipment on Wet or Dry Ice |
|---|---|
| Immunoassay Kit Format | Assay Activity Kit |
| Content And Storage | 96-well plate(s), standard, detection reagent, assay buffer, substrate, and lot-specific technical data sheet. Store at –20°C. |
| For Use With (Equipment) | Microplate Reader |
| Target Kit Named | Superoxide Dismutase (SOD) |
Invitrogen™ Human Granzyme B ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | 6.8% |
| Intraassay CV | 6.2 |
| Gene Symbol | GZMB |
| Total Assay Time | 2 hrs 30 mins |
| Sample Volume | Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | <0.43 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.7 to 480 pg/mL |
| Test Time | 1 hrs |
| Accession Number | P10144 |
| Regulatory Status | RUO |
| Target | Granzyme B |
| Kit Contents | Pre-coated 96 well plate,Standard,Detection antibody (monoclonal antibody to human Granzyme B),Biotin-Conjugate anti-human Granzyme B monoclonal antibody,Streptavidin-HRP,human Granzyme B Standard lyophilized,Wash Buffer Concentrate 20x,Dilution Buffer Concentrate 5x,Substrate Solution,Stop Solution,Adhesive Films |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3002 |
| Sample Type | Serum,Supernatant |
| Label or Dye | HRP |
Invitrogen™ Human MUC5AC ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| For Use With (Equipment) | Colorimetric Microplate Reader |
|---|---|
| Gene ID (Entrez) | 4586 |
| Interassay CV | <10% |
| Intraassay CV | <10 |
| Assay Sensitivity | 0.19 ng/mL |
| Assay Range | 0.31 to 20 ng/mL |
Invitrogen™ Human ALKP ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | ALPL |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 2 mIU/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 2 to 500 mIU/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | Akp2; Akp-2; Alkaline phosphatase 2; alkaline phosphatase 2, liver; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase precursor (EC 3.1.3.2); alkaline phosphatase tissue non-specific isoform; alkaline phosphatase tissue non-specific isoform/TNS-AP; alkaline phosphatase, biomineralization associated; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; Alpl; APTNAP; AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney isozyme; liver/bone/kidney-type alkaline phosphatase; MGC161443; MGC167935; PHOA; tissue-nonspecific alkaline phosphatase; tissue-nonspecific ALP; Tissue-nonspecific ALP alkaline phosphatase; TNAP; TNSALP; TNS-AP |
| Regulatory Status | RUO |
| Target | ALPL |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 249 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |