Immunoassay Reagents and Kits
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R&D Systems™ Human Total IL-18/IL-1F4 Quantikine ELISA Kit
Human Total IL-18/IL-1F4 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
Invitrogen™ PKA (Protein Kinase A) Colorimetric Activity Kit
Assay PKA (Protein Kinase A) Activity in Cell Lysates, Tissue Extracts and Buffer Samples.
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| Shipping Condition | Approved for shipment on Wet or Dry Ice |
|---|---|
| Immunoassay Kit Format | Assay Activity Kit |
| Content And Storage | 96-well plate(s), standard, detection reagent, assay buffer, substrate, and lot-specific technical data sheet. Store at –20°C. |
| For Use With (Equipment) | Microplate Reader |
| Target Kit Named | PKA (Protein Kinase A) |
Invitrogen™ Human IFN gamma ELISA Kit
ELISA
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 6.0% |
| Intraassay CV | 5.5 |
| Incubation Time | 2.75 hr. |
| Gene Symbol | IFG,IFI |
| Total Assay Time | 2 hrs 45 mins |
| Sample Volume | Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | <4 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 15.6 to 1000 pg/mL |
| Marker Gene | IFN-γ |
| For Use With (Application) | Colorimetric Detection |
| Test Time | 1 hrs 20 mins |
| Accession Number | P01579 |
| Regulatory Status | RUO |
| Target | IFN gamma |
| Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3458 |
| Sample Type | Serum,Supernatant |
| Label or Dye | HRP |
R&D Systems™ Human Fibronectin Quantikine ELISA Kit
a 4.5 hour solid-phase ELISA designed to measure human SDF-1 alpha in cell culture supernates and platelet-poor plasma.
Invitrogen™ Human HIF1A ELISA Kit
ELISA
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| Measurement | Absorbance at 450 nm minus absorbance (at 550 nm) |
|---|---|
| Protein Family | Transcription factors |
| Immunoassay Kit Format | Colorimetric ELISA (TMB substrate) with Plates |
| Host Species | Human |
| Target Species | Rat,Mouse |
| Conjugate | HRP |
| Intraassay CV | <10 |
| Substrate Type | TMB |
| Total Assay Time | 4 hrs |
| Sample Volume | Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL |
| Assay Sensitivity | 30 pg/mL |
| Research Discipline | Immunology |
| For Use With (Application) | Protein Assays and Analysis |
| Test Time | 1 hrs 20 mins |
| Detection Method | Colorimetric |
| Target | HIF1A |
| Specificity | Specific to human HIF-1 A and shows cross-reactivity with mouse and rat HIF-1 A |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| Measuring Range | 450 to 550 nm |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Label or Dye | HRP |
| Interassay CV | <10% |
| Gene Symbol | HIF-1-alpha,HIF-1 A,HIF-1 alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78 |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 81.92 to 20,000 pg/mL |
| Accession Number | Q16665 |
| Regulatory Status | RUO |
| Precision | Intra-assay CV <10%; inter-assay CV <10% |
| Product Type | ELISA |
| Gene ID (Entrez) | 3091 |
| Sample Type | Cell Lysates,Plasma,Serum |
Invitrogen™ Human Arginase-1 ELISA Kit
ELISA
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 8.5% |
| Intraassay CV | 6.6% |
| Gene Symbol | ARG1 |
| Total Assay Time | 3 hr 30 min |
| Sample Volume | Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL |
| Assay Sensitivity | 20.81 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 78.1-5000 pg/mL |
| Test Time | 1 hr 20 min |
| Accession Number | P05089 |
| Regulatory Status | RUO |
| Target | Arginase 1 |
| Kit Contents | Pre-coated 96 well plate, Standard (2 vials), Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, Streptavidin-HRP, Wash Buffer, Substrate Solution, Stop Solution, Adhesive Plate Covers,Standard (2 vials),Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,Streptavidin-HRP,Wash Buffer,Substrate Solution,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 383 |
| Sample Type | Cell Lysates,Plasma,Serum |
| Label or Dye | Biotin |
Invitrogen™ Malondialdehyde (MDA) Colorimetric Assay Kit
These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.
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Invitrogen™ Triiodothyronine (T3) Competitive ELISA Kit
ELISA
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Description | Triiodothyronine (T3) Competitive ELISA Kit |
| Interassay CV | 13.4% |
| Intraassay CV | 6.3 |
| Total Assay Time | 2 hrs 30 mins |
| Sample Volume | Fecal Extract 100 μL, Plasma 100 μL, Serum 100 μL, Supernatant 100 μL, Urine 25 μL |
| Assay Sensitivity | 37.4 pg/mL |
| Storage Requirements | -20°C |
| Research Discipline | Metabolism |
| Assay Range | 78.125 to 5000 pg/mL |
| For Use With (Application) | ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti |
| Test Time | 1 hrs 20 mins |
| Detection Method | Fluorescence |
| Regulatory Status | RUO |
| Target | Triiodothyronine (T3) |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,HRP-Conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Sample Type | Fecal Extract,Plasma,Serum,Supernatant,Urine |
| Label or Dye | HRP |
R&D Systems™ Human VEGF Quantikine ELISA Kit
Human VEGF Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
| Specificity | Natural and Recombinant Human VEGF. This assay also recognizes Recombinant Human VEGF165b. |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Gene ID (Entrez) | 7422 |
| Sample Type | Cell Culture Supernatant,Plasma (EDTA, Citrate, Heparin),Serum |
| Sample Volume | Cell Culture Supernates (200uL),Serum (100uL),EDTA Plasma (100uL),Heparin Plasma (100uL),Citrate Plasma (100uL) |
| Synonym | MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor |
| Assay Sensitivity | 5 pg/mL (Cell Culture Supernates), 9 pg/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma) |
| Storage Requirements | Store the unopened product at 2° to 8°C. Do not use past expiration date. |
| Assay Range | 15.60 to 1000 pg/mL (Cell Culture Supernates), 31.30 to 2000 pg/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma) |
| For Use With (Application) | ELISA |
| Cross Reactivity | Cross-species reactivity observed with 1 or more species tested. <0.5% cross-reactivity observed with available related molecules. |
Invitrogen™ Lactate Dehydrogenase (LDH) Colorimetric Activity Kit
These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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R&D Systems™ Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
Human IL-1 beta/IL-1F2 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
| Specificity | Natural and Recombinant Human IL-1 beta |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Product Type | Solid Phase Sandwich ELISA |
| Gene ID (Entrez) | 3553 |
| Sample Type | Cell Culture Supernatant,Plasma (EDTA, Citrate, Heparin),Serum |
| Sample Volume | Cell Culture Supernates (200uL),Serum (200uL),EDTA Plasma (200uL),Heparin Plasma (200uL),Citrate Plasma (200uL) |
| Synonym | catabolin, IL-1, IL1 beta, IL-1 beta, IL-1 b, IL1B, IL1-BETA, IL-1F2, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta |
| Assay Sensitivity | 1pg/mL |
| Storage Requirements | Store the unopened product at 2° to 8°C. Do not use past expiration date. |
| Assay Range | 3.90 - 250pg/mL |
| For Use With (Application) | ELISA |
| Cross Reactivity | <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules |
Invitrogen™ Human VEGF-A ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 4.3% |
| Intraassay CV | 6.2 |
| Gene Symbol | MVCD1,VEGF,VPF |
| Total Assay Time | 4 hrs 30 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 7.9 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 15.6 to 1,000 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 20 mins |
| Accession Number | P15692 |
| Regulatory Status | RUO |
| Target | VEGF |
| Kit Contents | Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 7422 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | Vascular endothelial growth factor A, VPF |
Invitrogen™ Human IL-12 p70 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 4.8% |
| Intraassay CV | 3 |
| Gene Symbol | CLMF,CLMF2,IL-12 B,IMD28,IMD29,NKSF,NKSF2,CLMF,IL-12 A,NFSK,NKSF1,P35 |
| Total Assay Time | 3 hrs 10 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 2.1 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 3.1 to 200 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 20 mins |
| Accession Number | P29459,P29460 |
| Regulatory Status | RUO |
| Target | IL-12 p70 |
| Kit Contents | Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Controls,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3592, 3593 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | Interleukin-12 subunit p70, CLMF, NKSF |
Invitrogen™ Mouse IgE Uncoated ELISA Kit
ELISA
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| Target Species | Mouse |
|---|---|
| Conjugate | HRP |
| Total Assay Time | 24 hrs 30 mins |
| Sample Volume | Plasma 10 μL, Serum 10 μL, Supernatant 50 μL |
| Assay Sensitivity | 4 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 4 to 250 ng/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 45 mins |
| Regulatory Status | RUO |
| Target | Mouse IgE |
| Kit Contents | Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Avidin-HRP,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 380792 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | Immunoglobulin E |
Invitrogen™ ProcartaPlex™ Rat Cytokine & Chemokine Panel, 22plex
The ProcartaPlex Rat Cytokine & Chemokine Panel 22plex enables the study of immune function by analyzing 22 protein targets in a single well using Luminex xMAP technology.
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