Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (132,601)

Singleplex and Multiplex Bead Based Immunoassays (6,181)

ELISA Substrates and Reagents (2,340)

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Invitrogen™ Mouse IL-6 ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Interassay CV	6.7%
Intraassay CV	5.8%
Gene Symbol	Il6
Total Assay Time	3 hr 30 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity	<3 pg/mL
Storage Requirements	2-8°C
Assay Range	7.8-500 pg/mL
For Use With (Application)	Colorimetric
Test Time	1 hr 20 min
Accession Number	P08505
Regulatory Status	RUO
Target	IL-6
Kit Contents	Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision	<3 pg/mL
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16193
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Human TNF alpha ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	7.5%
Intraassay CV	4.4%
Incubation Time	4 hr.
Gene Symbol	Tnf
Total Assay Time	4 hr
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	1.7 pg/mL
Storage Requirements	2-8°C
Assay Range	15.6-1000 pg/mL
Marker Gene	TNF-α
For Use With (Application)	Colorimetric Detection
Test Time	1 hr 20 min
Accession Number	P01375
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	7124
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Human EPO ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	4.3%
Intraassay CV	6.2%
Gene Symbol	EPO
Total Assay Time	3 hr 30 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.14 mIU/mL
Storage Requirements	2-8°C
Assay Range	1.6-100 mIU/mL
Test Time	1 hr 20 min
Accession Number	P01588
Regulatory Status	RUO
Target	Epo
Kit Contents	Pre-coated 96 well plate, Standard (2 vials), Assay Buffer concentrate, Biotinylated Detection Antibody, Streptavidin-HRP, Wash Buffer, Substrate Solution, Stop Solution, Adhesive Plate Covers, Control High, Control Low,Standard (2 vials),Assay Buffer concentrate,Biotinylated Detection Antibody,Streptavidin-HRP,Wash Buffer,Substrate Solution,Stop Solution,Adhesive Plate Covers,Control High,Control Low
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	2056
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Human TNF alpha (Total) ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	8.1%
Intraassay CV	7.7%
Gene Symbol	Tnf
Total Assay Time	3 hr 10 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	5.0 pg/mL
Storage Requirements	2-8°C
Assay Range	23-1500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Accession Number	P01375
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	7124
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	Cachectin, Tumor necrosis factor alpha, DIF

Invitrogen™ Mouse FSH ELISA Kit

ELISA

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Protein Family	Glycoprotein hormone
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Mouse
Conjugate	HRP
Interassay CV	<10%
Intraassay CV	<10%
Gene Symbol	CGA, FSHB
Total Assay Time	3 hr 30 min
Assay Sensitivity	0.94 ng/mL
Storage Requirements	Refer to product documentation for component specific storage temperature.
Assay Range	1.56-100 ng/mL
Test Time	1 hr 20 min
Accession Number	P01216, Q60687
Regulatory Status	RUO
Target	Follicle Stimulating Hormone
Kit Contents	Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer,Standard,Biotinylated Detection Antibody,HRP Conjugate,Standard & Sample Diluent,Biotinylated Detection Antibody Diluent,HRP Conjugate Diluent,Wash Buffer,TMB Substrate,Stop Solution,Plate Sealer
Gene Alias	class 1 follicle-stimulating hormone beta polypeptide; class 2 follicle-stimulating hormone beta polypeptide; follicle - stimulating hormone subunit beta; follicle stimulating hormone beta; follicle stimulating hormone beta subunit; follicle stimulating hormone beta-subunit precursor; follicle stimulating hormone subunit beta; follicle stimulating hormone, beta polypeptide; follicle-stimulating hormone beta subunit; follicle-stimulating hormone beta-subunit; follicle-stimulating hormone subunit beta; follicule stimulating hormone, beta polypeptide; follitropin beta chain; Follitropin subunit beta; follitropin, beta chain; FSH; FSH-alpha; FSHB; FSH-B; Fshbeta; FSH-beta; HH24; LSH-alpha; prefollicle stimulating hormone beta chain; TSH-alphaclass 1 follicle-stimulating hormone beta polypeptide; class 2 follicle-stimulating hormone beta polypeptide; follicle - stimulating hormone subunit beta; follicle stimulating hormone beta; follicle stimulating hormone beta subunit; follicle stimulating hormone beta-subunit precursor; follicle stimulating hormone subunit beta; follicle stimulating hormone, beta polypeptide; follicle-stimulating hormone beta subunit; follicle-stimulating hormone beta-subunit; follicle-stimulating hormone subunit beta; follicule stimulating hormone, beta polypeptide; follitropin beta chain; Follitropin subunit beta; follitropin, beta chain; FSH; FSH-alpha; FSHB; FSH-B; Fshbeta; FSH-beta; HH24; LSH-alpha; prefollicle stimulating hormone beta chain; TSH-alpha;
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	12640, 14308
Sample Type	Plasma,Serum
Label or Dye	Biotin

Invitrogen™ Human IFN gamma ELISA Kit

ELISA

Pricing & Availability
Specifications

Measurement	Absorbance at 450 nm minus absorbance (at 550 nm)
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Human
Conjugate	HRP
Description	Human IFN gamma ELISA Kit
Interassay CV	<12%
Intraassay CV	<10%
Gene Symbol	IFNG
Total Assay Time	3 hr
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	<2 pg/mL
Storage Requirements	2-8°C
Assay Range	25.6-1000 pg/mL
Test Time	1 hr 20 min
Accession Number	P01579
Regulatory Status	RUO
Target	IFN gamma
Specificity	No significant cross-reaction with with human IL-1 A, IL-1ß, IL-2, IL-3, IL-6, IL-7, IL-8, TNFa or GM-CSF
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision	Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3458
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Human VEGF ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	8.1%
Intraassay CV	4.7%
Incubation Time	4 hr.
Gene Symbol	VEGFA
Total Assay Time	4 hr
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	<5 pg/mL
Storage Requirements	2-8°C
Assay Range	23.4-1500 pg/mL
Marker Gene	VEGF
For Use With (Application)	Colorimetric Detection
Test Time	1 hr 20 min
Accession Number	P15692
Regulatory Status	RUO
Target	VEGF
Kit Contents	Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	7422
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Human IL-6 ELISA Kit, High Sensitivity

ELISA

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Protein Aliases	B-cell differentiation factor, B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IL-6, Interferon beta-2, interleukin BSF-2, Interleukin-6
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Human
Conjugate	HRP
Interassay CV	6%
Intraassay CV	4.9%
Gene Symbol	Il6
Total Assay Time	4 hr
Assay Sensitivity	0.03 pg/mL
Storage Requirements	2-8°C
Assay Range	0.08-5.0 pg/ml
Test Time	1 hr 20 min
Accession Number	P05231
Regulatory Status	RUO
Target	IL-6
Kit Contents	Pre-coated microwell plate, Biotinylated Detection Antibody, Streptavidin-HRP, Standard, Control (High), Control (Low), Sample Diluent, Assay Buffer Concentrate, Amplification Reagent I, Amplification Reagent II, Wash Buffer Concentrate, Substrate Solution, Stop Solution , Adhesive Plate Covers,Biotinylated Detection Antibody,Streptavidin-HRP,Standard,Control (High),Control (Low),Assay Buffer Concentrate,Amplification Reagent I,Amplification Reagent II,Wash Buffer Concentrate,Substrate Solution,Stop Solution,Adhesive Plate Covers
Gene Alias	B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6; BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	3569
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

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R&D Systems™ Human IL-10 Quantikine ELISA Kit

Human IL-10 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	IL10
Assay Sensitivity	3.9 pg/mL
Storage Requirements	Store the unopened product at 2°C to 8°C. Do not use past expiration date.
Assay Range	7.80 to 500 pg/mL
For Use With (Application)	ELISA
Target	IL-10
Specificity	Natural and Recombinant Human IL-10
Product Type	Solid Phase Sandwich ELISA
Gene ID (Entrez)	3586
Sample Type	Cell Culture Supernatant,Citrate,Plasma (EDTA, Citrate, Heparin),Serum
Synonym	CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL10A, IL-10 mgC126451, interleukin 10, interleukin-10, TGIF
Cross Reactivity	<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules

Invitrogen™ Testosterone High Sensitivity Competitive ELISA Kit

ELISA

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Protein Aliases	17b-Hydroxyandrost-4-ene-3-one, 17b-Testosterone, Testosterone
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Chemical
Conjugate	HRP
Interassay CV	<11%
Intraassay CV	<6%
Total Assay Time	2 hr 30 min
Assay Sensitivity	2.97 pg/mL
Storage Requirements	-20°C
Assay Range	7.8-1000 pg/mL
Test Time	1 hr 20 min
Regulatory Status	RUO
Target	Testosterone
Kit Contents	Pre-coated 96 well plate, Standard, Antibody, HRP-Conjugated Detection Antibody, Assay Buffer Concentrate, Wash Buffer, TMB Substrate, Stop Solution, Adhesive Plate Covers, Dissociation Reagent,Standard,Antibody,HRP-Conjugated Detection Antibody,Assay Buffer Concentrate,Wash Buffer,TMB Substrate,Stop Solution,Adhesive Plate Covers
Gene Alias	Androderm; C19H28O2; Mertestate; Sustanon; Testoderm; TestosteronAndroderm; C19H28O2; Mertestate; Sustanon; Testoderm; Testosteron;
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Sample Type	Fecal Extract,Plasma,Serum,Urine
Label or Dye	HRP

R&D Systems™ Human Granzyme B Quantikine ELISA Kit

Human Granzyme B Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability

Invitrogen™ Human IDO ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10%
Gene Symbol	Ido1
Total Assay Time	4 hr 45 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.81 ng/mL
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	0.819-200 ng/mL
Test Time	1 hr 20 min
Accession Number	P14902
Regulatory Status	RUO
Target	IDO
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3620
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Rat Growth Hormone ELISA Kit

ELISA

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Rat
Conjugate	HRP
Interassay CV	5%
Intraassay CV	4.8%
Substrate Type	Adaptor Proteins
Gene Symbol	Gh1
Total Assay Time	4 hr
Assay Sensitivity	<0.5 ng/mL
Storage Requirements	2-8°C
Assay Range	0.63-20 ng/mL
Test Time	1 hr 20 min
Accession Number	P01244
Regulatory Status	RUO
Target	Growth Hormone
Kit Contents	Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Gene Alias	dog growth hormone; GH; Gh1; GHB1; GHB5; GHN; GH-N; Growth hormone; growth hormone 1; growth hormone 12; growth hormone B1; growth hormone B5; growth hormone precursor; HGH; hGH-N; IGHD1B; pituitary growth hormone; rHGH; RNGHGP; Somatotropindog growth hormone; GH; Gh1; GHB1; GHB5; GHN; GH-N; Growth hormone; growth hormone 1; growth hormone 12; growth hormone B1; growth hormone B5; growth hormone precursor; HGH; hGH-N; IGHD1B; pituitary growth hormone; rHGH; RNGHGP; Somatotropin; Gh1
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	24391
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Human IGF-1 ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10%
Gene Symbol	IGF1
Total Assay Time	4 hr 45 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.1 ng/mL
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	0.1-30 ng/mL
Test Time	1 hr 20 min
Accession Number	P05019
Regulatory Status	RUO
Target	IGF1
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3479
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

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R&D Systems™ Human/Canine/Porcine Insulin Quantikine ELISA Kit

Human/Canine/Porcine Insulin Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability

Immunoassay Reagents and Kits

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R&D Systems™ Human IL-10 Quantikine ELISA Kit GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

R&D Systems™ Human Granzyme B Quantikine ELISA Kit Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

R&D Systems™ Human/Canine/Porcine Insulin Quantikine ELISA Kit Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

R&D Systems™ Human IL-10 Quantikine ELISA Kit

R&D Systems™ Human Granzyme B Quantikine ELISA Kit

R&D Systems™ Human/Canine/Porcine Insulin Quantikine ELISA Kit