Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (131,835)

Singleplex and Multiplex Bead Based Immunoassays (5,857)

ELISA Substrates and Reagents (2,333)

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Invitrogen™ Human IFN gamma ELISA Kit

ELISA

Pricing & Availability
Specifications

Measurement	Absorbance at 450 nm minus absorbance (at 550 nm)
Immunoassay Kit Format	Colorimetric ELISA (TMB substrate) with Plates
Target Species	Human
Conjugate	HRP
Description	Human IFN gamma ELISA Kit
Interassay CV	<12%
Intraassay CV	<10
Gene Symbol	IFG,IFI
Total Assay Time	3 hrs
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	<2 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	25.6 to 1000 pg/mL
Test Time	1 hrs 20 mins
Accession Number	P01579
Regulatory Status	RUO
Target	IFN gamma
Specificity	No significant cross-reaction with with human IL-1 A, IL-1ß, IL-2, IL-3, IL-6, IL-7, IL-8, TNFa or GM-CSF
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision	Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3458
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

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R&D Systems™ Human Leptin Quantikine ELISA Kit

Human Leptin Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability
Specifications

Specificity	Natural and Recombinant Human Leptin
Target Species	Human
Conjugate	HRP
Product Type	Solid Phase Sandwich ELISA
Gene ID (Entrez)	3952
Sample Type	Cell Culture Supernatant,Plasma (EDTA, Citrate, Heparin),Serum
Sample Volume	Cell Culture Supernates (100uL),Serum (10uL),EDTA Plasma (10uL),Heparin Plasma (10uL),Citrate Plasma (10uL)
Synonym	FLJ94114, LEP, leptin, leptin (murine obesity homolog), leptin (obesity homolog, mouse), OB, Obese protein, obese, mouse, homolog of, Obesity factor, OBOBS
Assay Sensitivity	7.8pg/mL
Storage Requirements	Store the unopened product at 2° to 8°C. Do not use past expiration date.
Assay Range	15.60 - 1000pg/mL
For Use With (Application)	ELISA
Cross Reactivity	<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules

Invitrogen™ Human SARS-CoV-2 RBD ELISA Kit

ELISA

Pricing & Availability
Specifications

For Use With (Equipment)	Colorimetric Microplate Reader
Interassay CV	<12%
Intraassay CV	<10
Assay Sensitivity	4.5 pg/mL
Assay Range	6.14 to 1500 pg/mL

Invitrogen™ Mouse IL-6 ELISA Kit

ELISA

Pricing & Availability
Specifications

Protein Aliases	IL-6, B-cell hybridoma growth factor, Interleukin HP-1
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Mouse
Conjugate	HRP
Interassay CV	8.9%
Intraassay CV	5%
Gene Symbol	Il6
Total Assay Time	3 hr 30 min
Assay Sensitivity	6.5 pg/mL
Storage Requirements	2-8°C
Assay Range	31.3-2000 pg/mL
Test Time	1 hr 20 min
Accession Number	P08505
Regulatory Status	RUO
Target	IL-6
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Gene Alias	B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6; IL-6
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	16193
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Human Amyloid beta 42 ELISA Kit, Ultrasensitive

ELISA

Pricing & Availability
Specifications

For Use With (Equipment)	Colorimetric Microplate Reader
Gene ID (Entrez)	351
Interassay CV	7.5%
Intraassay CV	8.6
Assay Sensitivity	<1 pg/mL
Assay Range	1.56 to 100 pg/mL

Invitrogen™ Cytokine 25-Plex Human Panel

Specifically designed for quantifying human cytokines and chemokines in serum, plasma, and tissue culture supernatant

Pricing & Availability

Invitrogen™ Human Aggrecan (PG) ELISA Kit

ELISA

Pricing & Availability
Specifications

For Use With (Equipment)	Colorimetric Microplate Reader
Gene ID (Entrez)	176
Interassay CV	5.6%
Intraassay CV	2.1
Assay Sensitivity	0.9 ng/mL
Assay Range	10 to 250 ng/mL
For Use With (Application)	Colorimetric Detection

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R&D Systems™ Mouse C-Reactive Protein/CRP Quantikine ELISA Kit

For the quantitative determination of mouse C-Reactive Protein (CRP) concentrations in cell culture supernates, tissue lysates, serum, and plasma

Pricing & Availability

Invitrogen™ Human Arginase-1 ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	8.5%
Intraassay CV	6.6%
Gene Symbol	ARG1
Total Assay Time	3 hr 30 min
Sample Volume	Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL
Assay Sensitivity	20.81 pg/mL
Storage Requirements	2-8°C
Assay Range	78.1-5000 pg/mL
Test Time	1 hr 20 min
Accession Number	P05089
Regulatory Status	RUO
Target	Arginase 1
Kit Contents	Pre-coated 96 well plate, Standard (2 vials), Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, Streptavidin-HRP, Wash Buffer, Substrate Solution, Stop Solution, Adhesive Plate Covers,Standard (2 vials),Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,Streptavidin-HRP,Wash Buffer,Substrate Solution,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	383
Sample Type	Cell Lysates,Plasma,Serum
Label or Dye	Biotin

Invitrogen™ Malondialdehyde (MDA) Colorimetric Assay Kit

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

Pricing & Availability

Invitrogen™ Human Hemoglobin ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10
Gene Symbol	HBA1
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements	2°C to 8°C
Assay Range	1.5 to 400 ng/mL
Test Time	1 hrs 20 mins
Accession Number	alpha one globin; alpha-1 globin; alpha-1-globin; alpha-2 globin chain; Alpha-globin; ALPHA-GLOBIN GENE; delta globin; Haemoglobin; Hb; HBA1; HbA1c; HBA2; HBA-T3; HBH; hemoglobin alpha 1 globin chain; Hemoglobin alpha chain; Hemoglobin subunit alpha; hemoglobin subunit alpha 1; hemoglobin, alpha 1
Regulatory Status	RUO
Target	Hemoglobin
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3039, 3043
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

Invitrogen™ Human IL-8 ELISA Kit

ELISA

Pricing & Availability
Specifications

For Use With (Equipment)	Colorimetric Microplate Reader
Gene ID (Entrez)	3576
Interassay CV	3.7%
Intraassay CV	4.2
Assay Sensitivity	0.7 pg/mL
Assay Range	7 to 750 pg/mL
For Use With (Application)	Colorimetric

Invitrogen™ Human TNF alpha (Total) ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	8.1%
Intraassay CV	7.7
Gene Symbol	DIF-alpha,TNFA,TNFSF2,TNLG1F,TNF
Total Assay Time	3 hrs 10 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	5.0 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	23 to 1,500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Accession Number	P01375
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	7124
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Cachectin, Tumor necrosis factor alpha, DIF

Invitrogen™ Human VEGF ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	8.1%
Intraassay CV	4.7
Incubation Time	4 hr.
Gene Symbol	MVCD1,VEGF,VPF
Total Assay Time	4 hrs
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	<5 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	23.4 to 1500 pg/mL
Marker Gene	VEGF
For Use With (Application)	Colorimetric Detection
Test Time	1 hrs 20 mins
Accession Number	P15692
Regulatory Status	RUO
Target	VEGF
Kit Contents	Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	7422
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

Invitrogen™ Human EGF ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	5.3%
Intraassay CV	4.4
Incubation Time	4 hr.
Gene Symbol	HOMG4,URG
Total Assay Time	4 hrs
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL, Urine 10 μL
Assay Sensitivity	<1 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	3.9 to 250 pg/mL
Marker Gene	EGF
For Use With (Application)	Colorimetric Detection
Test Time	1 hrs 20 mins
Accession Number	P01133
Regulatory Status	RUO
Target	EGF
Kit Contents	Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	1950
Sample Type	Plasma,Serum,Supernatant,Urine
Label or Dye	HRP

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Immunoassay Reagents and Kits

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R&D Systems™ Human Leptin Quantikine ELISA Kit GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

R&D Systems™ Mouse C-Reactive Protein/CRP Quantikine ELISA Kit GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

R&D Systems™ Human Leptin Quantikine ELISA Kit

R&D Systems™ Mouse C-Reactive Protein/CRP Quantikine ELISA Kit